Proteolytic processes in organism of different age rats exposed to xenoestrogens

Abstract

Endocrine disrupting chemicals (EDCs) are a group of compounds that affect the endocrine system, frequently found in everyday products and epidemiologically associated with several diseases. The human population is now ubiquitously exposed to EDCs in daily life. The main way of getting xenoestrogens to the body is the contaminated food. The effects of xenoestrogens on the proteolytic processes of different age rats were determination. The experiments were conducted on Wistar rats exposed to exogenous estrogen for 45 days. At the beginning of the experiment 3-month-old pubertal animals and 6-month-old sexually mature rats were involved. The research materials were organ tissue and blood serum of the rats. The objects were indexes of activity of trypsin and its obligatory inhibitors α1 - antitrypsin (α1-AT) and α2-macroglobulin (α2-MG), cysteine cathepsins B and L, the molecules of middle mass (MMM) level. In summary, the eating food contaminated by exoestrogens led to changes in the proteolytic system and the development of endogenous intoxication, which are also organ-specific and dependent on the age of the animals: a higher level of activity of the inhibitory link and the content of MMM was observed in rats in the puberty period, which leads to a decrease in the potential of the protective mechanisms of the organism and can become a trigger dysfunctional systems of natural detoxification and biotransformation. Inhibition of apoptosis is the main consequence found in the body of experimental rats. This phenomenon can lead to processes that inhibit one of the main mechanisms that reject damaged cells from the population. Females who were in puberty were more susceptible to dietary synthetic estrogens. In contrast to adult animals of the same sex, whose indicators indicate the importance of age characteristics of the body for the ability to perceive the effects of xenoestrogens. Rats became less sensitive to the effects of these substances with age. The difference in experimental animals was due to changes in the rate of detoxification pathway reactions, and not in the metabolism of estrogens entering the body, in particular, with food.